Phlebotomy and Blood Analysis for Fish and Invertebrates

Instructors:  Jill Arnold, MS, MLS (ASCP)CM, National Aquarium, Shayne Boylan, DVM, South Carolina Aquarium, Johnny Shelley, DVM, Private Firm, Roy Yanong, DVM, University of Florida, Pilar J. Nelson, MS, National Aquarium, Mark Fast, MS, PhD, Atlantic Veterinary College

The workshop will begin on September 2nd in the afternoon from 1 pm to 4:30 pm and will take place at the Atlantic Veterinary College – University of Prince Edward Island
Blood cell morphology varies among teleost and elasmobranch species.  While some cells resemble those found in birds and reptiles, additional cell types are present.  Multiple nomenclature systems lead to difficulty in interpretation of reported results and for application when performing WBC differentials.  Circulating cells in some invertebrates have been described, however hematology assays are not commonly included for animals, such as crustaceans and horseshoe crabs often housed in zoos and aquariums.  The aims for this continuing education workshop include an overview of blood cell types found in fish and shellfish and the reported nomenclature systems, phlebotomy sites, blood collection, anticoagulants, and hematology assay methods.  In addition to lecture information, participants will have the opportunity to have hands-on practice in blood sampling, slide preparation and staining, hemocytometer cell counts and select chemistry tests using point-of-care devices.

  • 1:00 pm – 1:50 pm:  Blood cell morphology of fish and invertebrates – Jill Arnold
  • 1:50 pm – 2:40 pm:  Laboratory techniques for hematology and clinical chemistry –                 Jill Arnold
  • 2:40 pm – 2:50 pm:  Coffee Break
  • 3:00 pm – 5 pm:        Hands-on opportunity to collect blood samples from fish and invertebrates, prepare and stain blood slides, perform cell counts using a hemocytometer and use point-of-care devices to measure select assays.

This workshop will be restricted to 30 participants, who will work in pairs.  Selection will be on a first come basis, and opens along with conference registration in January 2018.